Introduction to Metabolomics for the Microbiologist course - September 2018
19-21 September 2018 (Wednesday - Friday)
Birmingham Metabolomics Training Centre, School of Biosciences, University of Birmingham
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This three-day course introduced the attendees to how untargeted metabolomics can be applied to study microbial systems in academic and industrial research. The course provided an overview of the metabolomics pipeline from experimental design to sample preparation and data acquisition to data analysis/interpretation. The course was led by experts in the field of metabolomics and included lectures, hands-on laboratory sessions in sample preparation and data acquisition and computer workshops focused on data processing and data analysis.
Topics covered were:
- introduction to metabolomics, both targeted and untargeted approaches
- experimental design and the importance of quality control samples in untargeted metabolomics
- analytical strategies applied in metabolomics with a focus on mass spectrometry
- hands-on laboratory sessions focused on sample preparation and to include metabolic quenching and extraction procedures, intracellular and exometabolome samples and polar and non-polar extraction methods
- hands-on laboratory sessions focused on sample analysis for untargeted metabolomics studies using an ACQUITY UPLC coupled to a Xevo QToF mass spectrometer
- hands-on workshop focused on data processing and data analysis
- hands-on workshop focused on an introduction to metabolite identification
- question and answer session with the experts.
The course was aimed at individuals with minimal experience of applying metabolomics in their research and no or limited experience of using a liquid chromatography - mass spectrometer. The attendees will have left the course understanding, and being able to apply, the metabolomics pipeline in their research.
This course was free, including travel and accommodation, for PhD students and selected post-doctoral researchers funded by NERC (subject to availability).